Ccute and chronic toxicity tests with fish

Fish acute and chronic toxicity tests with several different fish species (e.g. rainbow trout, zebrafish, common carp, fathead minnow and medaka) are offered.

In summary, the following tests are conducted in our laboratories:

  • Acute toxicity tests
  • Fish embryo tests
  • Fish early life stage toxicity test
  • Bioaccumulation in Fish: Aqueous and Dietary Exposure (with fish, oysters and other species)
  • Endocrine studies 
  • Feeding Studies
  • Pharmakokinetic studies

Depending on the properties of the test item static, semi-static or flow through test designs are applied. Various technical options for dosing of flow-through systems are available.  Tests on bioaccumulation are offered with fish, oysters and other species.

Acute toxicity studies:

The tests are performed according to OECD 203 or OPPTS 850.1075 with the following fish species:

  • Rainbow trout (Onchorynchus mykiss)
  • Zebrafish (Danio rerio)
  • Fathead minnow (Pimephales promelas)
  • Carp (Cyprio carpio)
  • Medaka (Oryzias latipes)
  • and several other species on demand

We also offer the Fish Embryo Acute Toxicity (FET) Test according to OECD 236.

Long-term toxicity studies:

  • Fish juvenile growth test (OECD 215)
  • Bioconcentration test (OECD 305)
  • Early life stage toxicity test (OECD 210)
  • Short term toxicity testing on embryo and sac-fry stages (OECD 212)
  • Fish juvenile growth test (OECD 215)

For standard test design zebra fish and rainbow trout will be used. Other species can be offered on demand.

General facts:

  • Zebrafish, fathead and medaka are cultured and reared in-house.
  • Local suppliers are sourced for rainbow trout, carp and others.
  • All tests can be performed static, semi-static or under flow-through conditions.
  • Depending on test item properties several different pumping devices for application are available like e.g. peristaltic-, HPLC- and diluter pumps.
  • Tests can be performed with purified tap water or with reconstituted water according to ISO 6341. Fully automated water provision is available.
  • All labs are state-of-the-art temperature controlled.