Eurofins Regulatory Science Services provides a broad array of GLP-compliant testing services required to demonstrate safety, distribution, and metabolism of chemical substances.

Our scientists apply decades of experience and state-of-the-art instrumentation to deliver studies with rigorous methods, reliable data, and repeatable results. Their services include study protocol development, preparation of radiolabeled test substance formulations, homogenization of collected samples, total radioactive residue determination, and the extraction, radio-chromatographic profiling, isolation and identification of metabolites. Our team of organic synthesis chemists is ready to prepare radiolabeled test substances, stable labeled analogs, and/or postulated metabolites as reference substances for your study.

Plant Metabolism

Eurofins Regulatory Science Services conducts GLP compliant plant metabolism studies that include target crop and confined rotational crop studies with radiolabeled test substances.

Our Study Directors coordinate the in-life portion of all plant metabolite projects, growing and caring for the test plants throughout each study in addition to preparing radiolabeled test substance formulations, performing the appropriate type of application of the radiolabeled test substance, and collecting tissue samples at intervals from application through harvest. The in-life portion of plant metabolism studies can be conducted in outdoor plots or inside one of our greenhouses. Collected study samples are then processed and analyzed by our scientists to determine the nature of residues in plant matrices.

Other Metabolism Studies

Eurofins Regulatory Science Services also conducts GLP-compliant metabolism studies across a variety of species. We perform comparisons of metabolites of test substances using in vitro incubation with heptatocytes/S9 fractions. Our Study Directors oversee the in-life portion of these studies at our laboratories. Our scientists then process and analyze their collected samples to determine the nature of the residues in these matrices.

Our metabolism testing capabilities include:

  • Nature of residue
  • Confined accumulation in rotational crops
  • Bioaccumulation in fish and metabolism in fish
  • Rat ADME
    • Metabolism of test substance in rats
    • Pharmacokinetics
    • Tissue distribution
    • Bile cannulation
    • Dermal absorption of pesticides
  • Multi-site GLP experience/expertise
  • Comprehensive plant/confined rotational crop studies
  • Metabolism studies: multiple species
  • In-house custom synthesis of radiolabeled compounds (14C, 3H), stable labeled analog compounds (13C, 15N, 2H), and metabolites
  • Full analytical support
    • Determination of total radioactive residues (TRR)
    • Extraction of radiolabeled species
    • Mass balance
    • Extractions and hydrolysis of conjugates/separation of matrix components
    • Profiling and identification by comparison to authentic standards
    • Confirmation of identification of metabolites by GC-MS and high resolution LC-MS/MS and NMR
    • Proposal of metabolic pathways